In-Lab SEC-SAXS for Structual Investigation of Protein Samples
- SAXS can be used to investigate biological macromolecules especially in monodisperse solutions.
- However liquid bio-solutions involve structurally different molecules and complexes in dynamic equilibria.
- SEC – Size Exclusion Chromatography has evolved as a technique to mitigate this by separating the molecules in time.
- However this limits the measurements time window which put huge
requirements on the X-ray source.
- With MetalJet source in the Xenocs BioXolver system it is possible to
perform protein in-lab SEC-SAXS even for very diluted solutions containing
less than 1 mg of sample.
Application note kindly provided by Application Scientist at Xenocs SAS using a BioXolver equipped with Metaljet X-ray source.
You find the Appl. note in full here.
Bucciarelli, S. et al. Size-exclusion chromatography small-angle X-ray scattering (SEC-SAXS) of water soluble proteins on a laboratory instrument, J. Appl. Crystallogr. 2018