Methods based on scattering and diffraction utilize the radiation that changes direction when interacting with the object. This typically provides information on length scales smaller than what can be directly imaged. In small angle X-ray scattering, samples are analyzed to obtain information about sizes, shapes and orientations of internal structures. The analyzed features are mostly in the size range 1-200 nm. For structures down to the atomic scale, X-ray diffraction is a very powerful technique to analyze crystalline samples. For example, the atomic structures of entire proteins can be resolved.


小角X線散乱法 (SAXS)




In-Lab SEC-SAXS for Structual Investigation of Protein Samples

  • SAXS can be used to investigate biological macromolecules especially in monodisperse solutions.

    Xenocs BioXolver equipped with MetalJet

  • However liquid bio-solutions involve structurally different molecules and complexes in dynamic equilibria.
  • SEC – Size Exclusion Chromatography has evolved as a technique to mitigate this by separating the molecules in time.
  • However this limits the measurements time window which put huge
    requirements on the X-ray source.
  • With MetalJet source in the Xenocs BioXolver system it is possible to
    perform protein in-lab SEC-SAXS even for very diluted solutions containing
    less than 1 mg of sample.


Application note kindly provided by Application Scientist at Xenocs SAS using a BioXolver equipped with Metaljet X-ray source.
You find the Appl. note in full here.
Bucciarelli, S. et al. Size-exclusion chromatography small-angle X-ray scattering (SEC-SAXS) of water soluble proteins on a laboratory instrument, J. Appl. Crystallogr. 2018.



Bruker AXSのアプリケーション科学者は、ラットテール腱からの非常に細い繊維の一連のSAXS散乱プロットを記録するためにMetalJet(70Wで200W)を備えたNANOSTAR装置を使用しました。最先端の密封されたマイクロフォーカスチューブと比較して、50倍以上の強度ゲインを提供します。



南洋理工大学のA * STAR、ルイビル大学、ロザリンド・フランクリン医科学大学、慶煕大学の研究者は、ミトコンドリアカルシウムイオン輸送の重要な調節因子であるBcl-xLタンパク質の研究にMetalJet搭載のSAXS装置を使用しました。タンパク質を弱い中性洗剤で処理し、2つの単量体間のヘリックスα6-α8の三次元ドメイン交換(3DDS)から生じる二量体の形成の間に、研究が行われました。

Sci. Rep. 5, 10609 (2015), S. Rajan, M. Choi, Q. T. Nguyen, H. Ye, W. Liu, H. T. Toh, C. B. Kang, N. Kamariah, C. Li, H. Huang, C. White, K. Baek, G. Grüber, H. S. Yoon.




Sensors and Actuators A: Physical, 2016, 247, 87-95, K. Vegso, M. Jergel, P. Siffalovic, M. Kotlar, Y. Halahovetsa, M. Hodasa, M. Pellettaa, E. Majkovaa




Macromolecules, 2016, 49 (18), 6731–6742, P. Yang, O. O. Mykhaylyk, E. R. Jones, S. P. Armes.


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